PCR Cloning Protocols (9781617372810)

PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.

Product details

• Format Paperback | 439 pages
• Dimensions 155 x 235 x 25.91mm | 1,470g
• Publication date 10 Nov 2010
• Publisher Humana Press Inc.
• Publication City/Country Totowa, NJ, United States
• Language English
• Edition Revised
• Edition Statement Softcover reprint of hardcover 2nd ed. 2002
• Illustrations note XIV, 439 p.
• ISBN10 1617372811
• ISBN13 9781617372810

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